Thomas scientific is the premier online source for lab supplies, equipment, instruments and chemicals all shipped direct to you quickly and safely. Hitrap capto iex selection kit offers a fast, simple. Epoxyactivated sepharose 6b ge healthcare life sciences. A chromatographic matrix containing hydrophobic groups, binds proteins from aqueous solutions to different extents depending on the protein structures and a range of controllable factors including concentrations of salts, ph, temperature and organic solvents fig 2. Hitrap columns, capto iex selection kit, ge healthcare vwr. The kit consists of seven different ion exchange resins prepacked in hitrap 1 ml.
Save time submit your details once and make multiple inquiries. The five hitrap 1 ml columns are also included in hitrap iex selection kit together with sp sepharose xl and q sepharose xl. Hitrap iex selection kit 17600233 hitrap q hp 17115401 hitrap butyl ff 175701 mono s 550gl 17516801. Hitrap iex selection kit is used for screening of the optimal ge ion exchange resin for specific application and development work. Ge healthcare hitrap iex selection kit includes seven 1ml iex. Purification antibody purification handbook 18103746 gel. The columns are delivered with a stopper at the inlet and a snapoff end at the outlet. Check that the carousel is positioned correctly and that there are enough clean tubes. The developed phgradient iex was applied as an analytical tool for the selection of phrelated conditions in an ionexchange chromatographic capture of a mab from hybridoma ccs. Media and column selection refer to ion exchange selection guide code no. Related media available in hitrap iex selection kit although it does not include q or sp sepharose high performance media, the hitrap iex selection kit will be of interest to many potential users of these two ion exchangers.
Q sepharose fast flow and sp sepharose fast flow are also available. Hitrap iex selection kit offers a fast, simple, and convenient way to decide which ion exchanger or ion exchange ligand is best for a given application. Media selection hitraptm iex selection kit, including seven different iex media, is used for fast screening of iex ligands and for method optimization. With the use of the fplc, one can separate and characterize protein mixture as well as pure proteins. The separation is based on the reversible interaction between a charged protein and an oppositely charged chromatography resin. The kit consists of seven different ion exchange media based on sepharose fast flow and sepharose xl. Ge healthcare hitrap capto iex selection kit hitrap capto iex selection kit packings for chromatography columns chromatography. The seven different hic media are prepacked in ready to use 1 ml hitrap columns. It was shown that the investigated mab can optimally be captured by bindandelute mode cex at ph 4. Hitrap capto ion exchange selection kit cytiva formerly ge. Click on the chromatography method you are interested in to jump to the corresponding selection guide. Protein and peptide purification hebrew university of. A scale up was performed to reproduce the purification step with the fraction that demonstrated antiproliferative activity.
Download free ge user manuals, owners manuals, instructions, warranties and installation guides, etc. Selection of better resolving columns improved resolution and integration of the challenging separation new system suitability test solution was incorporated into method revision product understanding allowed justification of distinct shelflife specification for pre main peak. Thomas scientific is a registered contractor for the u. Designed for use with syringe, peristalic pump, or chromatography systems such as akta design for convenient media screening and optimization studies.
Hitrap capto iex selection kit is designed for screening the optimal capto ion exchange or ge multimodal chromatography resin prior to scaleup. Filled with stationary phase compositions or resins, the prepacked options reduce the preparing time required. Iex is a binding technique, independent of sample volume. Principle of ion exchange chromatography ion exchange iex chromatography can separate molecules. Biomolecules are purified using chromatography techniques that separate them according to differences in their specific properties, as shown in figure 1. Data file 18114048 ae ion exchange chromatography hitrap. Sec size exclusion chromatography iex ion exchange chromatography ac affinity chromatography ge format guide type of purification. Hitrap iex selection kit is a tool which offers a fast, easy and convenient. Hitrap iex selection kits contain sepharose fast flow and sepharose xl in combination with seven different ion exchange ligands.
Epoxyactivated sepharose 6b epoxyactivated sepharose 6b is a preactivated resin for immobilization of various ligands including sugars through coupling of hydroxy, amino or thiol groups on the ligand to sepharose 6b via a atom hydrophilic spacer arm order product. The kit consists of seven different ion exchange media. Selection kit for iex resins in hitrap columns cytiva formerly ge. Using technology similar to a water softenertrace amounts of minerals or other contamination are condensate polishing unit from the system before such contamination becomes concentrated enough to cause condensate polishing unit by depositing minerals inside pipes, or within precisionengineered devices condensate polishing unit as boilerssteam generatorsheat.
Hitraptm iex selection kit offers a fast, simple, and convenient way to decide which ion exchanger or ion exchange ligand is best for a given application. Ge hitrap q hp users manual page 18 free pdf download. Ion exchange chromatography iex separates proteins with differences in surface charge to give highresolution separation with high sample loading capacity. Hitrapconvenient protein purification fisher scientific. Hitrap protein ag, histrap ff crude, hitrap talon superdex, mono s, mono q, resource, capto qs, iex hic selection kits prepacked columns hitrap can use without system, hiscreen, hiprep. See also ion exchange columns and media guide, 18112731. The hitrap hic selection kit consists of seven hydrophobic interaction chromatography hic media with different hydrophobic characteristics. Microsoft powerpoint purifier instructional material. Ion exchange columns and media hebrew university of.
Hitrap iex selection kit hitrap sp ff, 1 ml hitrap cm ff, 1 ml hitrap q ff, 1 ml hitrap deae ff, 1 ml hitrap anx ff high sub, 1 ml hitrap q xl, 1 ml hitrap sp xl, 1 ml hitrap iex selection kit consists of seven hitrap 1 ml columns, prepacked with different ion exchangers. The kit consists of seven different ion exchange media prepacked in hitrap 1 ml columns. Property technique size gel filtration gf, also called size exclusion charge ion exchange chromatography iex hydrophobicity hydrophobic i nteraction chromatography hic. Use hitrap iex selection kit for media scouting and method optimization. Table 1 lists the characteristics of hitrap columns. Hitrap iex selection kit consists of seven hitrap columns, prepacked with different sepharose fast flow ion exchange chromatography media for screening of the most kiit media and conditions to use for specific application and development work.
Ge healthcare hitrap capto iex selection kit fisher scientific. Sample preparation samples should be at the same ph and ionic strength as the starting buffer, and free from. Use small prepacked columns for media scouting and method optimization, to increase efficiency in method development e. Hitrap capto iex selection kit is designed for convenient media screening and optimization studieschromatography columns separate molecules within mixtures during gas or liquid methods. Epoxyactivated sepharose 6b is a preactivated resin for immobilization of various ligands including sugars through coupling of hydroxy, amino or thiol groups on the ligand to. The kit provides you with the possibility to screen for the most appropriate hic media to use for specific application and development work. Documentation is easy as result files contain a complete record of a run, including method, curve data and run log. Combine chromatography techniques to optimize your protein. Several ion exchange chromatography iex and hydrophobic interaction chromatography hic resins were tested using hitrap iex selection kit and hitrap hic selection kit ge healthcare, baie durfe, qc, canada. Hitrap sp hp, 1 ml and 5 ml hitrap q hp, 1 ml and 5 ml. Federal government and provides supplies and equipment to over 25 agencies including the center for disease control, the food and drug administration, health and. Related resins available in hitrap iex selection kit although it does not include q or sp sepharose high performance resins, the hitrap iex selection kit will be of interest to many potential users of these two ion exchangers.
Ge healthcare hitrap capto iex selection kit hitrap capto. Documentation is easy as result files contain a complete record of a run, including method, curve data and run. Prepacked columns from ge healthcare will ensure reproducible results and the highest performance. Ge healthcare your guide to chromatography media pure proteins today. Read and download ge dishwasher hitrap q hp users manual page 18 online. Select steepest gradient to give acceptable resolution at. Hitrap column characteristics the columns are made of biocompatible polypropylene that does not interact with biomolecules. Hitrap iex selection kit ge healthcare, 17600233 sigmaaldrich. Use hitrap iex selection kit for media scouting and method optimisation. Supply of iex column and matrix 1 68 19 p item iex tender. Hitrap capto iex selection kit ge healthcare, 28934388. Other ligands can be coupled through hydroxyl, amino or thiol groups.
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